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Objective@#To investigate whether silencing UCP2 can sensitize cervical cancer cell line Siha to radiation.@*Methods@#Siha cells were transfected with UCP2 siRNA and then irradiated by X-ray. The radiosensitivity of Siha cells was verified by colony formation, CCK-8, apoptosis and immunofluorescence assays. The mitochondrial membrane potential and the production of reactive oxygen species (ROS) were detected to further explore the related mechanism.@*Results@#RT-PCR and Western blot assays showed that the expression of UCP2 in Siha cells was increased after irradiation and the UCP2 siRNA successfully silenced the expression of UCP in cells. According to the survival curves, the D0, Dq, N and SF2 were 1.54, 1.31, 2.31 Gy and 0.52 for siUCP2 group, 2.50, 3.64, 4.30 Gy and 0.83 for blank control group, and 3.34, 2.16, 1.91 Gy and 0.69, for siNC group, respectively. The radiosensitivity enhancement ratio of silent group was 0.62 and 0.46, compared with blank control group and negative control group, respectively. The proliferative activity of cells in the silent group was lower than that in the control group (t=13.2, P<0.05). Apoptosis levels in the silent group were significantly higher than those in the control group after irradiation(t=3.14, P<0.05). At 4 h after irradiation, the ROS production in the silent group was significantly higher than that in the control group (t=19.10, P<0.05). At 24 h after irradiation, the mitochondrial membrane potential of Siha cells in the silent group was significantly lower than that in the control group (t=4.18, P<0.05).@*Conclusions@#The radiosensitivity of Siha cells is enhanced after UCP2 silencing, and thus UCP2 may applicable as a new target for radiosensitization of cervical cancer cells.
ABSTRACT
Objective To investigate whether silencing UCP2 can sensitize cervical cancer cell line Siha to radiation.Methods Siha cells were transfected with UCP2 siRNA and then irradiated by Xray.The radiosensitivity of Siha cells was verified by colony formation,CCK-8,apoptosis and immunofluorescence assays.The mitochondrial membrane potential and the production of reactive oxygen species (ROS) were detected to further explore the related mechanism.Results RT-PCR and Western blot assays showed that the expression of UCP2 in Siha cells was increased after irradiation and the UCP2 siRNA successfully silenced the expression of UCP in cells.According to the survival curves,the D0,Dq,N and SF2 were 1.54,1.31,2.31 Gy and 0.52 for siUCP2 group,2.50,3.64,4.30 Gy and 0.83 for blank control group,and 3.34,2.16,1.91 Gy and 0.69,for siNC group,respectively.The radiosensitivity enhancement ratio of silent group was 0.62 and 0.46,compared with blank control group and negative control group,respectively.The proliferative activity of cells in the silent group was lower than that in the control group (t =13.2,P＜0.05).Apoptosis levels in the silent group were significantly higher than those in the control group after irradiation (t=3.14,P＜0.05).At 4 h after irradiation,the ROS production in the silent group was significantly higher than that in the control group (t=19.10,P＜0.05).At 24 h after irradiation,the mitochondrial membrane potential of Siha cells in the silent group was significantly lower than that in the control group (t =4.18,P ＜ 0.05) Conclusions The radiosensitivity of Siha cells is enhanced after UCP2 silencing,and thus UCP2 may applicable as a new target for radiosensitization of cervical cancer cells.
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Objective To discuss the surgical treatment of intraductal papillary mucinous neoplasms of the pancreas (IPMN).Methods The clinical data of 42 IPMN cases undergoing surgical treatment in our hospital during the past 4 years were analyzed retrospectively.Results Routine CT or MRI examination was made in 42 cases,ERCP in 6 cases,ultrasonic endoscopy in 12 cases,fine needle biopsy by endoscopic ultrasonography in 4 cases.There were 12 cases of the main duct IPMN,24 cases of branch duct IPMN,6 cases of mixed IPMN.Pancreatoduodenectomy was performed in 24 cases;6 cases underuent laparoscopic central pancreatectomy;10 cases did distal pancreatectomy,2 cases did total pancreatectomy.Postoperative pathology revealed that the malignant transformation rate of main pancreatic duct type was 66.7％,that of BD-IPMN was 12.5％ and 66.7％ of Mix-IPMN.The postoperative complication rate was 26.2％.All cases were followed up for 1 month to 4 years,with no tumor recurrence.Conclusions Imaging examination combined with endoscopic ultrasonography is the main method for clinical diagnosis of IPMN.The malignant transformation rate of MD-IPMN and Mix-IPMN is high.Most of the BD-IPMN is hyperplasia and adenoma.